Detection of Bean Common Mosaic Virus in Bean Seeds by Immunocapture RT-PCR and DAS-ELISA Methods

Ultrastructural Localization of Bean Common Mosaic Virus in Dormant and Germinating Seeds of Phaseolus vulgaris

HOCH, H. C., and R. PROVVIDENTI. 1978. Ultrastructural localization of bean common mosaic virus in dormant and germinating seeds of Phaseolus vulgaris. Phytopathology 68: 327-330. The ultrastructural localization of bean common mosaic of undulated filaments. Following 48 hr of water imbibition virus (BCMV) was determined for mature dehydrated and and growth, meristem cells of the root apex were...

Sequence Analysis of Expressed cDNA of Bean Common Mosaic Virus RU1 Isolate

RNAi-mediated resistance to Bean golden mosaic virus in genetically engineered common bean (Phaseolus vulgaris).

Bean golden mosaic virus (BGMV) is transmitted by the whitefly Bemisia tabaci in a persistent, circulative manner, causing the golden mosaic of common bean (Phaseolus vulgaris L.). The characteristic symptoms are yellow-green mosaic of leaves, stunted growth, or distorted pods. The disease is the largest constraint to bean production in Latin America and causes severe yield losses (40 to 100%)....

Comparison of DAS-ELISA and RT-PCR Methods for the Detection of Prunus Necrotic Ringspot Virus (PNRSV)

Two methods (DAS-ELISA and RT-PCR) were compared for the detection of Prunus necrotic ringspot virus (PNRSV) in woody host Prunus mahleb isolated from Malatya. Total RNA extractions were made from serially diluted fresh leaf tissue, root, bark and one year old green bark tissue using silica-based method. Purified total RNA extracts were used as template for cDNA synthesis by reverse transcripti...

NL-3 K Strain Is a Stable and Naturally Occurring Interspecific Recombinant Derived from Bean common mosaic necrosis virus and Bean common mosaic virus.

ABSTRACT A strain of Bean common mosaic necrosis virus (BCMNV) from Idaho was identified by enzyme-linked immunosorbent assay using monoclonal antibodies and determined to be similar to the NL-3 D strain (of Drifjhout) by reaction of differential bean cultivars. However, this BCMNV strain (designated NL-3 K) caused earlier and more severe symptoms on bean plants representing host groups 0, 4, a...